Leukocyte Adherence Inhibition Test to the assessment of Immunoreactivity Against Cow’s Milk Proteins in Non—IgE-Mediated Gastrointestinal Food Allergy



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Submitted Mar 19, 2022
Published Apr 13, 2022
10.24018/clinicmed.2022.3.2.189

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Background:  The non—IgE-mediated food allergy syndromes are a group of conditions diagnosed mostly by clinical criteria and Oral Food Challenge tests (OFC).

Objective:  To evaluate the feasibility of the Leukocyte Adherence Inhibition Test (LAIT) to discriminate antigen-specific immunoreactivity in a group of patients with non—IgE-mediated cow’s milk allergy diagnosed by OFC and clinical criteria.

Methods:  Ex vivo challenge tests performed with cow’s milk proteins extracts were monitored by LAIT in two groups: A) the active group consisting of 38 patients presenting non—IgE-mediated gastrointestinal lactose-free cow’s milk hypersensitivity; B) the control group consisting of 29 cow’s milk tolerant individuals

Results:  The mean Leukocyte Adherence Inhibition (LAI) of the control group was 5.48%, and the mean LAI of the active group was 54.18%.  The non-parametric Wilcoxon-Mann-Whitney U test showed p < α = 0.05.

Conclusion:  The Leukocyte Adherence Inhibition Test is an ex vivo immunoassay able to predict individual non—IgE-mediated immunoreactivity against cow’s milk allergens and to act as a triage test to select food allergens to be prescribed in exclusion diets to perform OFC in patients with suspected non—IgE-mediated food allergies.

Keywords: Adult, allergy, antigen, food, cellular immunity, enterocolitis, hypersensitivity, immunoassay, leukocyte adherence inhibition test, milk

References

  1. Gell PGH, Coombs RRA. Classification of Allergic Reactions Responsible for Clinical Hypersensitivity and Disease. In: Gell PGH, Coombs RRA, editors. Clinical Aspects of Immunology. 2nd ed. Oxford: Blackwell Scientific Publications; 1968. p. 575-96.
     Google Scholar
  2. König B, Koch AN, Bellanti JA. Studies of mitochondrial and nuclear DNA released from food allergen-activated neutrophils. Implications for non-IgE food allergy. Allergy Asthma Proc. 2021;42(3):e59-e70.
     Google Scholar
  3. Lawless OJ, Bellanti JA, Brown ML, Sandberg K, Umans JG, Zhou L, et al. In vitro induction of T regulatory cells by a methylated CpG DNA sequence in humans: Potential therapeutic applications in allergic and autoimmune diseases. Allergy Asthma Proc. 2018;39(2):143-52.
     Google Scholar
  4. Bellanti JA. Genetics/epigenetics/allergy: The gun is loaded … but what pulls the trigger? Allergy Asthma Proc. 2019;40(2):76-83.
     Google Scholar
  5. Li D, Cheng J, Zhu Z, Catalfamo M, Goerlitz D, Lawless OJ, et al. Treg-inducing capacity of genomic DNA of Bifidobacterium longum subsp. infantis. Allergy Asthma Proc. 2020;41(5):372-85.
     Google Scholar
  6. Nowak-Węgrzyn A, Katz Y, Mehr SS, Koletzko S. Non-IgE-mediated gastrointestinal food allergy. J Allergy Clin Immunol. 2015;135(5):1114-24.
     Google Scholar
  7. Olivier CE. Food Allergy. J Allergy Ther. 2013;S3:004:1-7 doi:10.4172/2155-6121.S3-004.
     Google Scholar
  8. Leonard SA. Non-IgE-mediated Adverse Food Reactions. Curr Allergy Asthma Rep. 2017;17(12):84.
     Google Scholar
  9. Agyemang A, Nowak-Wegrzyn A. Food Protein-Induced Enterocolitis Syndrome: a Comprehensive Review. Clin Rev Allergy Immunol. 2019;57(2):261-71.
     Google Scholar
  10. Fernandes BN, Boyle RJ, Gore C, Simpson A, Custovic A. Food protein-induced enterocolitis syndrome can occur in adults. J Allergy Clin Immunol. 2012;130(5):1199-200.
     Google Scholar
  11. Caubet JC, Bencharitiwong R, Ross A, Sampson HA, Berin MC, Nowak-Wegrzyn A. Humoral and cellular responses to casein in patients with food protein-induced enterocolitis to cow's milk. J Allergy Clin Immunol. 2017;139(2):572-83.
     Google Scholar
  12. Tan JA, Smith WB. Non-IgE-mediated gastrointestinal food hypersensitivity syndrome in adults. J Allergy Clin Immunol Pract. 2014;2(3):355-7.
     Google Scholar
  13. Abdullah M. Irritable bowel syndrome: current review on pathophysiology and diagnostic aspects. Acta Med Indones. 2008;40(4):218-25.
     Google Scholar
  14. Harrington LK, Mayberry JF. A re-appraisal of lactose intolerance. Int J Clin Pract. 2008;62(10):1541-6.
     Google Scholar
  15. Greiwe J. Using oral food challenges to provide clarity and confidence when diagnosing food allergies. J Food Allergy. 2021;3(1):3-7.
     Google Scholar
  16. Olivier CE, Lorena SLS, Pavan CR, Santos RAPG, Lima RPS, Pinto DG, et al. Is it just lactose intolerance? Allergy Asthma Proc. 2012;33(5):432-6.
     Google Scholar
  17. Bicudo Mendonça R, Motta Franco J, Rodrigues Cocco R, Suano de Souza FI, Lopes de Oliveira LC, Saccardo Sarni RO, et al. Open oral food challenge in the confirmation of cow's milk allergy mediated by immunoglobulin E. Allergol Immunopathol. 2012;40(1):25-30.
     Google Scholar
  18. Calvani M, Bianchi A, Reginelli C, Peresso M, Testa A. Oral Food Challenge. Medicina (Kaunas, Lithuania). 2019;55(10):651.
     Google Scholar
  19. Bellanti JA, Settipane RA. The allergist and IgE: The realization that allergic diseases are not all IgE mediated. Allergy Asthma Proc. 2021;42(3):183-6.
     Google Scholar
  20. Settipane RA. Food allergy: Science of uncertainty and art of probability. J Food Allergy. 2021;3(1):1-2.
     Google Scholar
  21. Powell GK. Milk- and soy-induced enterocolitis of infancy: Clinical features and standardization of challenge. J Pediatrics. 1978;93(4):553-60.
     Google Scholar
  22. Olivier CE, Pinto DG, Teixeira APM, Santana JLS, Santos RAPGS, Lima RPS. Contribution of the Leukocyte Adherence Inhibition Test to the Evaluation of Cellular Immunoreactivity against Latex Extracts for Non—IgE-Mediated Latex-Fruit-Pollen Syndrome in Allergic Candidates to Exclusion Diets and Allergic Desensitization. Eur J Clin Med. 2022;3(1):1-7.
     Google Scholar
  23. Olivier CE, Pinto DG, Teixeira APM, Santana JLS, Santos RAPGS, Lima RPS. Contribution of the Leukocyte Adherence Inhibition Test for the evaluation of immunoreactivity against gluten extracts in non—IgE-mediated / non-autoimmune Gluten-Related Disorders. Eur J Clin Med. 2022;3(2):1-7.
     Google Scholar
  24. Olivier CE, Argentão DGP, Santos RAPG, Silva MD, Lima RPS, Zollner RL. Skin scrape test: an inexpensive and painless skin test for recognition of immediate hypersensitivity in children and adults. Open Allergy J. 2013;6:9-17.
     Google Scholar
  25. World Medical Association Declaration of Helsinki: ethical principles for medical research involving human subjects. JAMA. 2013;310(20):2191-4.
     Google Scholar
  26. International-Committee-of-Medical-Journal-Editors. Uniform requirements for manuscripts submitted to biomedical journals. International Committee of Medical Journal Editors. BMJ. 1991;302(6772):338-41.
     Google Scholar
  27. Olivier CE, Pinto DG, Lima RPS, Silva MDd, Santos RAPG, Teixeira APM, et al. Assessment of Immunoreactivity against Therapeutic Options Employing the Leukocyte Adherence Inhibition Test as a Tool for Precision Medicine. Eur J Clin Med. 2021;2(3):40-5.
     Google Scholar
  28. Kim H-Y. Statistical notes for clinical researchers: Nonparametric statistical methods: 1. Nonparametric methods for comparing two groups. Restor Dent Endod. 2014;39(3):235-9.
     Google Scholar
  29. Fay MP, Proschan MA. Wilcoxon-Mann-Whitney or t-test? On assumptions for hypothesis tests and multiple interpretations of decision rules. Stat Surv. 2010;4:1-39.
     Google Scholar
  30. Shek LPC, Bardina L, Castro R, Sampson HA, Beyer K. Humoral and cellular responses to cow milk proteins in patients with milk-induced IgE-mediated and non-IgE-mediated disorders. Allergy. 2005;60(7):912-9.
     Google Scholar
  31. Boyden S. The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes. J Exp Med. 1962;115:453-66.
     Google Scholar
  32. Butler HL, Byrne WJ, Marmer DJ, Euler AR, Steele RW. Depressed Neutrophil Chemotaxis in Infants with Cow's Milk and/or Soy Protein Intolerance. Pediatrics. 1981;67(2):264.
     Google Scholar
  33. Vanto T, Smogorzewska EM, Viander M, Kalimo K, Koivikko A. Leukocyte migration inhibition test in children with cow milk allergy. Allergy. 1987;42(8):612-8.
     Google Scholar
  34. Halliday WJ, Miller S. Leukocyte adherence inhibition: a simple test for cell-mediated tumour immunity and serum blocking factors. Int J Cancer. 1972;9(3):477-83.
     Google Scholar
  35. Bullen AW, Losowsky MS. Comparison of a leucocyte adherence test with the leucocyte migration inhibition test and skin reactivity to PPD. Clin Exp Immunol. 1978;31(3):408-13.
     Google Scholar
  36. Kuratsuji T. Studies on leukocyte adherence inhibition test. Part II. Clinical applications of LAI test to detect delayed type hypersensitivity in infants and children. Keio J Med. 1981;30(2):65-9.
     Google Scholar
  37. Kuratsuji T. Studies on leukocyte adherence inhibition test. Part I. Studies on mechanisms of leukocyte adherence inhibition. Keio J Med. 1981;30(2):53-63.
     Google Scholar
  38. Olivier CE, Lima RPS, Pinto DG, Santos RAPG, Silva GKM, Lorena SLS, et al. In search of a tolerance-induction strategy for cow's milk allergies: significant reduction of beta-lactoglobulin allergenicity via transglutaminase/cysteine polymerization. Clinics. 2012;67(10):1171-9.
     Google Scholar
  39. Olivier CE, Pinto DG, Teixeira APM, Santana JLS, Santos RAPG, Lima RPS. Immunoreactivity against Dermatophagoides pteronyssinus Assessed by the Leukocyte Adherence Inhibition Test in Patients with Intrinsic Atopic Dermatitis and Correlated “Intrinsic” Non–IgE-mediated Allergic Conditions. Eur J Clin Med. 2021;2(6):45-50.
     Google Scholar
  40. Thomson DMP. Assessment of immune status by the leukocyte adherence inhibition test. New York: Academic Press; 1982. xvii, 380 p. p.
     Google Scholar
  41. Halliday WJ. Historical Background and Aspects of the Mechanism of Leukocyte Adherence Inhibition. Cancer Res. 1979;39(2):558-63.
     Google Scholar
  42. Appelboom T, Famaey JP, Gortz R, Wybran J. Effect of levamisole on leukocyte adherence inhibition. Agents Actions. 1981;11(6-7):604-5.
     Google Scholar
  43. Fink A, Bibi H, Eliraz A, Tabachnik E, Bentwich Z. Leukotrienes (LTC4, LTD4) confer glass non-adherence on leukocytes of asthmatic individuals. Dependency on cyclooxygenase products and calcium ion. Immunol Lett. 1985;10(6):319-23.
     Google Scholar
  44. Fink A, Shahin R, Eliraz A, Bibi H, Berkenstadt H, Levin S, et al. Interferon modulates the leukotriene C4-induced non-adherence properties of leukocytes: acquisition of an asthmatic phenotype. Immunol Lett. 1985;10(3-4):159-63.
     Google Scholar
  45. Iwabuchi K, Yamashita T. Platelet-derived neutrophil adherence-inhibiting factor in humans. Blood. 1990;76(11):2368-73.
     Google Scholar
  46. Powell AE, Birch RE, Murrell H, Sloss AM. Cell populations in leucocyte adherence inhibition: requirement for T lymphocytes with IgG Fc receptors. Immunol. 1982;46(4):689-96.
     Google Scholar
  47. Kotlar HK, Sanner T. Role of circulating antibodies in the humoral leukocyte adherence inhibition response of lung and breast cancer patients. Cancer Lett. 1980;11(1):11-9.
     Google Scholar
  48. Olivier CE, Lima RPdS, Pinto DG, Santos RAPGd. The Plasma Preincubation with Papain Before the Assay Suggests that a Gell and Coombs Type II Reaction is Been Demonstrated by the Leukocyte Adherence Inhibition Test. Biom J Sci Tech Res. 2021;36(3):28647 - 55.
     Google Scholar
  49. Gocki J, Bartuzi Z. Role of immunoglobulin G antibodies in diagnosis of food allergy. Postepy dermatologii i alergologii. 2016;33(4):253-6.
     Google Scholar
  50. Xie Y, Zhou G, Xu Y, He B, Wang Y, Ma R, et al. Effects of Diet Based on IgG Elimination Combined with Probiotics on Migraine Plus Irritable Bowel Syndrome. Pain Res Manag. 2019;2019:7890461.
     Google Scholar
  51. Zuo XL, Li YQ, Li WJ, Guo YT, Lu XF, Li JM, et al. Alterations of food antigen-specific serum immunoglobulins G and E antibodies in patients with irritable bowel syndrome and functional dyspepsia. Clin Exp Allergy. 2007;37(6):823-30.
     Google Scholar
  52. Beyer K, Teuber SS. Food allergy diagnostics: scientific and unproven procedures. Curr Opin Allergy Clin Immunol. 2005;5(3).
     Google Scholar
  53. Patterson MF, Dorris SL. Food Allergy: Unproven diagnostics and therapeutics. J Food Allergy. 2020;2(1):91-4.
     Google Scholar
  54. Alkhateeb AF. Foods Causing Highest IgG Immune Response in Saudi Arabia. Ann Res Rev Biol. 2020;35(3):115-27.
     Google Scholar
  55. Zapatero L, Alonso E, Fuentes V, Martínez MI. Oral desensitization in children with cow's milk allergy. J Investig Allergol Clin Immunol. 2008;18(5):389-96.
     Google Scholar
  56. Olivier CE, Lima RPS, Argentão DGP, Silva MDd, Santos RAPG, Pensuti M, et al. Group-specific Multi-allergen Sublingual/Swallow Immunotherapy Improves the Quality of Life of Polysensitized Children and Adults with Allergic Rhinitis. J Allergy Ther. 2013;4(5):148. doi: 10.4172/2155-6121.1000148.
     Google Scholar
  57. Berin MC, Agashe C, Burks AW, Chiang D, Davidson WF, Dawson P, et al. Allergen-specific T cells and clinical features of food allergy: Lessons from CoFAR immunotherapy cohorts. J Allergy Clin Immunol. 2021.
     Google Scholar
  58. Tong AW, Burger DR, Finke P, Barney C, Vandenbark AA, Vetto RM. Assessment of the mechanism of the leukocyte adherence inhibition test. Cancer Res. 1979;39(2 Pt 2):597-603.
     Google Scholar
  59. Dunn IS, Halliday WJ. Interactions between T and B lymphocytes and macrophages in the production of leukocyte adherence inhibition factor. Cell Immunol. 1980;52(1):48-61.
     Google Scholar
  60. Haw WY, Polak ME, McGuire C, Erlewyn-Lajeunesse M, Ardern-Jones MR. In vitro rapid diagnostic tests for severe drug hypersensitivity reactions in children. Ann Allergy Asthma Immunol. 2016;117(1):61-6.
     Google Scholar


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