Black Soybean (Glycine max (L.) Merr.) 30% Ethanol Extract Cream Inhibited The Increase of Tyrosinase Enzyme Expression and The Amount of Melanin Same Effective as 4% Hydroquinone Cream on Guinea Pig (Cavia porcellus) Skin

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  •   Yanvi Johan

  •   Anak Agung Gde Putra Wiraguna

  •   Ni Nyoman Ayu Dewi

Abstract

Background: Hyperpigmentation is one of the signs of skin aging which is mostly caused by Ultraviolet B (UVB) rays exposure. The gold standard for hyperpigmentation therapy is hydroquinone (HQ) but its long-term use can cause side effects such as irritation, rebound phenomenon, and even ochronosis. Black soybeans contain active ingredients that can prevent the increase of tyrosinase enzyme and the amount of melanin so that it can be used as an alternative therapy for hyperpigmentation. This study aims to prove that the administration of 30% black soybean extract cream was as effective as 4% HQ cream in inhibiting the increase of tyrosinase enzyme expression and the amount of melanin in guinea pig skin exposed to UVB rays.


Methods: This study used a post-test only control group design method with 30 guinea pigs as samples. The sample was divided into three groups of 10 each. The P1 group was given the base cream, the P2 group was given the 4% HQ cream, while the P3 group was given the 30% black soybean extract cream. Each group was exposed to UVB rays with a total dose of 390mJ/cm2 for 2 weeks, after that the guinea pig skin tissue was taken and histopathological preparations were made for examination of tyrosinase enzyme expression and the amount of melanin. The data obtained were analyzed using one way ANOVA, then the differences between groups were analyzed using the Least Significant Difference (LSD) test.


Results: The results showed that the mean of the tyrosinase enzyme expression and the amount of melanin in the black soybean extract group P3 (12.880±3.327% and 3.16±1.486) were not significantly different from the HQ4% group P2 (9.690±2.176% and 2.18±1.117), while the mean of the base cream group P1 (25.78±5.392% and 12.080±3.150) was the highest among the three groups. The LSD test showed a significant difference between the base cream group (P1) and the other groups (p<0.01), but there was no significant difference between the 4% hydroquinone group (P2) and the 30% black soybean extract treatment group (P3) (p >0.05).


Conclusion: The conclusion of this study was that 30% black soybean ethanol extract cream inhibited the increase of tyrosinase enzyme expression and the amount of melanin as effective as 4% HQ cream on guinea pig skin exposed to UVB light.


Keywords: Black soybean, melanin, tyrosinase

References

Baumann L, Saghari S. Skin Pigmentation and Pigmentation Disorders. Cosmetic Dermatology. 2nd edition. New York: McGraw Hill. 2009B: 98-106.

Wiraguna AAGP. Aspek Anatomi dan Fisiologi Kulit. Denpasar : Udayana University Press. 2020.

Denat L, Kadekaro AL, Marrot L, Leachman SA, Abdel-Malek ZA. Melanocytes as instigators and victims of oxidative stress. Journal of Investigative Dermatology. 2014; 134(6): 1512-1518.

D'Orazio J, Jarrett S, Amaro-Ortiz A, Scott T. UV radiation and the skin. International Journal of Molecular Sciences. 2013; 14(6): 12222–12248.

Bruce S. Safety and efficacy of a novel multimodality hydroquinone-free skin brightener over six months. J Drugs Dermatol. 2013; 12(3): S27-31.

Fawwaz M, Muliadi DS, Muflihunna A. Kedelai Hitam (Glycine soja) terhidrolisis sebagai sumber flavonoid total. Jurnal Fitofarmaka Indonesia. 2017; 4(1): 194-198. Indonesian.

Sumardi D, Pancoro A, Yulia E, Musfiroh I, Prasetiyono J, Karuniawan A, et al. Potential of local black Soybean as a Source of the Isoflavones Daidzein and Genistein. International Food Reaserch Journal. 2017; 24(5): 2140-2145.

Wijaya V, Sriwidyani NP, Aman IGM. Glycine max (L) Merrit Extracts Inhibited the Enhancement of Tyrosinase Expression and the Increase in the Amount of Melanin on Cavia Porcellus Skin that is exposed to Ultraviolet B. International Journal of Science and Research (IJSR). 2020; 9(7): 1668 – 1671.

Lai J, Xin C, Zhao Y, Feng B, He C, Dong Y, et al. Study of active ingredients in black soybean sprouts and their safety in cosmetic use. Molecules. 2012; 17(10): 116669-11679.

Mukhtarini. Ekstraksi, Pemisahan Senyawa, dan Identifikasi Senyawa Aktif. Jurnal of Pharmacy. 2011; VII(2). Indonesian.

Carballo-Carbajal I, Laguna A, Romero-Gimenez J, Cuadros T, Bove J, Martinez-Vicente M, et al. Brain tyrosinase overexpression implicates age dependant neuromelanin production in Parkinson’s disease pathogenesis. Nature Communications. 2019; 10(1): 1-19.

Miot HA, Brianezi G, Tamega Ade A, Miot LD. Techniques of digital image analysis for histological quantification of melanin. An Bras Dermatol. 2012; 87(4): 608-611.

Hunyadi A. The mechanism(s) of action of antioxidants: From scavenging reactive oxygen/nitrogen species to redox signaling and the generation of bioactive secondary metabolites. Medicinal Research Reviews. 2019; 39(6): 2505-2533.

Banjarnahor SDS, Artanti N. Antioxidant properties of flavonoids. Medical Journal of Indonesia. 2014; 23(4): 239-244.

Waqas MK, Akhtar N, Rasul A, Rashid SU, Mustafa R, Khan BA, et al. In vivo evaluation of a cosmetic emulsion containing soybean extract for anti-aging. Tropical Journal of Pharmaceutical Research. 2014; 13(9): 1401-1406.

Gu C, Song X, Zao L, Pan S, Qin G. Purification and Characterization of Bowman-Birk Trypsin Inhibitor from Soybean. Journal of Food and Nutrition Research. 2014; 2(9): 546-550.

Pillaiyar T, Manickam M, Namasivayam V. Skin whitening agents: Medicinal chemistry perspective of tyrosinase inhibitors. Journal of Enzyme Inhibition and Medicinal Chemistry. 2017; 32(1): 403–425.

Zhou Q, Feng C, Ruan Z. Inhibitory Effect of a Genistein Derivative on Pigmentation of Guinea Pig Skin. RSC Advances. 2017; 7(13): 7914-7919.

Sanada E. Pemberian Krim Ekstrak Etanol Bunga Gemitir (Tagetes Erecta) Mencegah Peningkatan Kadar Tirosinase dan Ekspresi Melanin Kulit Marmut (Cavia porcellus) yang Dipapar Ultraviolet B (tesis). Denpasar : Universitas Udayana. 2020.

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How to Cite
Johan, Y., Wiraguna, A. A. G. P., & Dewi, N. N. A. (2022). Black Soybean (Glycine max (L.) Merr.) 30% Ethanol Extract Cream Inhibited The Increase of Tyrosinase Enzyme Expression and The Amount of Melanin Same Effective as 4% Hydroquinone Cream on Guinea Pig (Cavia porcellus) Skin. European Journal of Clinical Medicine, 3(3), 5–9. https://doi.org/10.24018/clinicmed.2022.3.3.212